Elevated expression of miR-423-5p in the plasma of patients with ischemic stroke
10.16352/j.issn.1001-6325.2025.12.1572
- VernacularTitle:miR-423-5p在缺血性卒中患者血浆中的表达升高
- Author:
Haipeng LI
1
;
Yifan LIU
;
Shan LI
;
Daohui CHEN
;
Jinglin LEI
;
Zheng HU
Author Information
1. 南华大学衡阳医学院 郴州市第一人民医院 转化医学研究所,湖南 郴州 423000;南华大学衡阳医学院 郴州市第一人民医院神经内科,湖南 郴州 423000
- Keywords:
miR-423-5p;
ischemic stroke;
biomarker;
apoptosis
- From:
Basic & Clinical Medicine
2025;45(12):1572-1579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance of miR-423-5p in ischemic stroke and its effects on SH-SY5Y cell apoptosis along with the underlying mechanisms.Methods Plasma samples were collected from three stroke-prone patients who subsequently developed acute ischemic stroke(AIS)within 24 hours.High-throughput sequencing was used to identify miR-423-5p as a key target.Venous blood samples were collected from 46 AIS pa-tients within 24 hours of onset and from 46 matched control cases.RT-qPCR was performed to measure the plasma expression of miR-423-5p in both groups.The diagnostic value of miR-423-5p for AIS was evaluated using receiver operating characteristic(ROC)curve.After overexpression and knockdown of miR-423-5p in SH-SY5Y cells,flow cytometry was performed to detect apoptosis in each group to analyze the potential relationship between miR-423-5p and SH-SY5Y cell apoptosis.Western blot was used to measure changes in the expression level of the ap-optotic protein cleaved caspase-3 and the anti-apoptotic proteins Bcl-2 and Bcl-xL.Results The expression of miR-423-5p in 3 AIS patients showed significant difference before and after onset.Plasma miR-423-5p expression was significantly elevated in AIS patients versus controls(P＜0.001).ROC analysis indicated its strong diagnostic potential for AIS.Overexpression of miR-423-5p increased the apoptosis rate and cleaved caspase-3 ex-pression of SH-SY5Y cells after OGD/R,and decreased the expression of Bcl-2 and Bcl-xL,while knocking down miR-423-5p had the opposite effect.Conclusions The expression of miR-423-5p is upregulated in the plasma of patients with ischemic stroke compared to healthy controls.Furthermore,miR-423-5p promotes OGD/R-induced apoptosis in SH-SY5Y cells by increasing the level of cleaved caspase-3 and suppressing the expression of Bcl-2 and Bcl-xL.
