Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury
10.16352/j.issn.1001-6325.2025.12.1557
- VernacularTitle:骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤
- Author:
Wen WEN
1
;
Chenxi LIU
;
Shuangjing CHEN
;
Xiaojiong LU
;
Zhitao JIN
;
Zheng ZHANG
Author Information
1. 锦州医科大学火箭军特色医学中心研究生培养基地 心血管内科,北京 100088
- Keywords:
exosomes;
hypoxia/reoxygenation;
oxidative stress;
mitochondria;
apoptosis
- From:
Basic & Clinical Medicine
2025;45(12):1557-1564
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanisms of bone marrow mesenchymal stem cell(BMMSC)-derived exosomes(BMMSC-Exo)on hypoxia/reoxygenation(H/R)-induced injury in rat cardiomyocyte cell line(H9c2).Methods BMMSC-Exosomes were isolated by ultracentrifugation.The cells were divided into three groups:control,H/R,and H/R+BMMSC-Exo(H/R+Exo).A hypoxia/reoxygenation(H/R)injury model was es-tablished by exposing cells to 12 hours of hypoxia followed by 6 hours of reoxygenation.Flow cytometry was used to detect cell apoptosis,DHE staining was used to assess cellular ROS levels,JC-1 immunofluorescence staining was used to evaluate mitochondrial membrane potential,and Western blot was used to detect mitochondrial autophagy-re-lated proteins.Results BMMSC-Exo treatment significantly alleviated oxidative stress,restored mitochondrial mem-brane potential,reduced mitochondrial autophagy levels,and effectively decreased cardiomyocyte apoptosis.Conclu-sions Bone marrow mesenchymal stem cell-derived exosomes alleviate H/R-induced cardiomyocyte injury.
