Decreased expression of SFXNs in renal tissues of mouse models of acute and chronic kidney disease
10.16352/j.issn.1001-6325.2025.12.1541
- VernacularTitle:急性和慢性肾脏病小鼠模型中肾组织SFXNs表达下降
- Author:
Li GAO
1
;
Siyi WANG
;
Minjing ZHANG
;
Lin ZHAO
;
Zheming XU
;
Gensheng ZHANG
;
Jieping YAN
Author Information
1. 浙江省人民医院 杭州医学院附属人民医院 临床药学中心 药学部,浙江 杭州 310014;浙江大学医学院附属儿童医院 国家儿童健康与疾病临床医学研究中心,浙江 杭州 310052
- Keywords:
cisplatin;
ischemia reperfusion;
acute kidney injury;
chronic kidney disease;
sideroflexins
- From:
Basic & Clinical Medicine
2025;45(12):1541-1547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of iron autophagy-mitochondrial ferric ion transport protein families(SFXNs)in acute kidney injury(AKI)and chronic kidney disease(CKD)mouse models induced by cisplatin(Cis)and ischemia reperfusion(IR).Methods C57BL/6 mice were randomly divided into control group(control),Cis-AKI group,Cis-CKD group,sham-operated group(sham),IR-AKI group,and IR-CKD group.Serum and kidney tissue samples were collected from mice.Serum creatinine(Cr)and blood urea nitrogen(BUN)levels were detected.Pathological changes in renal tissues were observed by HE staining.Western blot was used to detect the expression of renal SFXNs and kidney injury related proteins.Results Compared with the control or sham group,the levels of BUN and Cr in the serum of the model group were significantly increased(P＜0.05),the renal tissue showed significant pathological damage,with the kidney injury molecule-1(KIM-1),neutrophil gelatinase-associated lipocalin(NGAL),and pro-apoptotic protein Bax significantly upregulated(P＜0.05),while the anti-apoptotic protein Bcl-2 was significantly downregulated(P＜0.05).Compared to the control or sham group,the Cis-AKI group showed a significant downregulation of SFXN4(P＜0.05);The SFXN4 and SFXN5 subtypes were significantly downregulated in the IR-AKI group and Cis-CKD group(P＜0.05);All five subtypes of SFXN in the IR-CKD group were significantly downregulated(P＜0.05).Conclusions Cis or IR in-duces renal tissue damage and tubular mitochondrial injury in mice and affects the expression of SFXN family pro-teins,suggesting their potential role in renal injury of animal models.
