miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells
10.16352/j.issn.1001-6325.2025.11.1457
- VernacularTitle:miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡
- Author:
Zonghu JIA
1
;
Qun JIN
;
Shufang HAN
;
Yuhong HU
;
Changzhen REN
;
Yunping LI
;
Wenyan LIU
Author Information
1. 锦州医科大学中国人民解放军联勤保障部队第九六○医院研究生培养基地心内科,山东济南 250031
- Keywords:
miR-374c-5p;
human umbilical vein endothelial cells(HUVECs);
hydrogen peroxide(H2O2);
oxidative stress;
apoptosis
- From:
Basic & Clinical Medicine
2025;45(11):1457-1462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of miR-374c-5p on hydrogen peroxide(H2O2)-induced apoptosis of human umbilical vein endothelial cells(HUVECs).Methods HUVECs were cultured in vitro and the harvested cells were divided into four groups:control group,H2O2 group,H2O2+miR-374c-5p mimics trans-fection group,and H2O2+miR-374c-5p inhibitor transfection group.Cell activity was assessed by CCK-8 prolifer-ation rate assay,apoptosis was detected by TUNEL staining microscopy.Expression of miR-374c-5p and Fas mRNA by RT-qPCR,and Fas protein in HUVECs by was detected by Western blot.Results Proliferation of HUVECs was significantly inhibited(P＜0.001);H2O2 was significantly increased as compared with the H2O2 in-tervention group(P＜0.001);Proliferation in H2O2+miR-374c-5p inhibitor transfection group was significantly increased as compared to H2O2 intervention group(P＜0.001).Conclusions miR-374c-5p protectes the HUVECs against apoptosis induced by H2O2.
