Downregulation of Talin1 inhibits migration of pulmonary arterial smooth muscle cells(PASMCs)induced by serum of rat models with HPS
10.16352/j.issn.1001-6325.2025.11.1409
- VernacularTitle:下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移
- Author:
Yang CHEN
1
;
Jing WEN
;
Lan SHI
;
Yong YANG
;
Bin YI
;
Lin CHEN
Author Information
1. 重庆市第七人民医院(重庆理工大学附属中心医院)麻醉科,重庆 400054
- Keywords:
hepatopulmonary syndrome;
pulmonary arterial smooth muscle cells;
Talin 1;
Integrin β1
- From:
Basic & Clinical Medicine
2025;45(11):1409-1414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of Talin1 in the migration of rat pulmonary artery smooth muscle cells(PASMCs)induced by the serum from rats with hepatopulmonary syndrome(HPS).Methods Twenty male Spra-gue-Dawley(SD)rats were used as HPS rat models by chronic common bile duct ligation,the serum was collected from abdominal aorta.PASMCs were seeded in 6-well and 24-well plates and randomly divided into control group and HPS group.The cells were transfected with Talin1 or control siRNA.The normal rat serum or HPS rat serum with a final concentration of 5％was added respectively.At 24 hours after cell transfection or at 24 hours(T1),48 hours(T2)and 72 hours(T3)of cell incubation,the protein expression of Talin1 and active Integin β1 in PASMCs were determined by Western blot;The migration of PASMCs was measured by Transwell chamber(T1)and scratch assay(T1 to T3).Results Compared to control group,with the extension of the stimula-tion time in the HPS group,the expression of Talin1 protein was upregulated,and the migration of PASMCs was gradually enhanced(P＜0.05);Talin1 siRNA effectively silenced the Talin1 gene;The expression of ac-tive Integin β1 protein and the migration of PASMCs in the HPS group+si control were enhanced(P＜0.05);Compared with the HPS group+si control,the expression of active Integin β1 protein and the migration of PASMCs in the HPS group+siTalin1 were significantly inhibited(P＜0.05).Conclusions Downregulating ex-pression of Talin1 protein inhibits migration of PASMCs and expression of active Integin β1 protein induced by the serum from HPS rats.
