Down-regulation of METTL3 reduces Hcy-induced macrophage M1 polarization and foaminess
10.16352/j.issn.1001-6325.2025.10.1341
- VernacularTitle:下调METTL3降低Hcy诱导的巨噬细胞M1极化和泡沫化
- Author:
Yu LIANG
1
;
Junhong LI
;
Jianqiong WANG
;
Li WEI
;
Jie JIANG
;
Mingyuan WANG
;
Min SU
Author Information
1. 云南省第一人民医院/昆明理工大学附属医院 检验科,云南 昆明 650034
- Keywords:
homocysteine;
macrophage polarization;
macrophage foam;
METTL3;
N6-methyladenosine
- From:
Basic & Clinical Medicine
2025;45(10):1341-1349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of macrophage polarization and foaminess by homocysteine(Hcy)and its potential underlying mechanisms.Methods ELISA and flow cytometry were used to detect the effect of Hcy treatment on the polarization of macrophages.The contents of various forms of intracellular cholesterol were detected,and the effects of Hcy on intracellular lipid accumulation and ox-LDL uptake were evaluated by oil red O staining and Dil-oxLDL.RT-qPCR and Western blot were used to detect mRNA and pro-tein expression of key genes modified by N6-methyladenosine(m6 A).Results Hcy promoted M1 polarization of macrophages and ox-LDL-induced foam macrophages and promoted ox-LDL uptake as well as intracellular lipid accumulation.In addition,Hcy upregulated methyltransferase like 3(METTL3)expression,and the tendency of Hcy to promote macrophage M1 polarization and foaminess was markedly reduced after inhibition or knockdown of METTL3 expression.Conclusions Hcy significantly promotes macrophage M1 polarization and foaminess,an effectthat may be attenuated by METTL3 silencing.
