Sigma-1 receptor expression in rat brain tissue is correlated with brain injury after cardiopulmonary resuscitation
10.16352/j.issn.1001-6325.2025.09.1200
- VernacularTitle:大鼠脑组织sigma-1受体表达与心肺复苏后脑损伤相关
- Author:
Haiyan ZHAO
1
;
Yijie WANG
;
Rong LIU
;
Jilin YANG
;
Ting LI
;
Xiaolin ZHU
;
Jiahong QIN
Author Information
1. 昆明医科大学第一附属医院 老年重症医学科,云南 昆明 650032
- Keywords:
cardiopulmonary resuscitation after cardiac arrest;
global cerebral ischemia-reperfusion injury;
sigma-1 receptor;
mito-chondria;
endoplasmic reticulum stress
- From:
Basic & Clinical Medicine
2025;45(9):1200-1207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of sigma-1 receptor(Sig-1R)on brain function in rats after cardiopulmo-nary resuscitation and its protective role in brain injury.Methods Rats were randomly assigned to four groups with 20 in each:sham-operated control(sham group),6-hour post-resuscitation(PR 6 h group),12-hour post-resuscitation(PR 12 h group)and 24-hour post-resuscitation(PR 24 h group).In the latter three groups,cardiac arrest was induced by as-phyxiation,and cardiopulmonary resuscitation was performed 6 minutes after cardiac arrest.The rats were scored for neu-rological deficits at 6,12 and 24 hrs after resuscitation,respectively;after that,the rats were executed,and the expres-sion of Sig-1R protein,mitochondrial function index,and endoplasmic reticulum stress index apoptosis index were detec-ted by Western blot and immunohistochemistry.The correlation between Sig-1R and mitochondrial,endoplasmic reticulum stress and apoptosis indexes was evaluated.Results Compared with the sham-operated group,the rats in test group showed a gradual decrease in neurological deficit scores,Sig-1R protein expression,brain tissue adenosine triphos-phate(ATP)concentration and mitochondrial membrane potential(MMP)levels at 6,12,and 24 hrs of PR(P＜0.05);CHOP protein,activated cleaved caspase-12 and cleaved caspase-3 protein expression were consistently elevated(P＜0.05).In addition,Sig-1R was negatively correlated with brain tissue endoplasmic reticulum stress and apoptosis(P＜0.05)but positively correlated with mitochondrial membrane potential level(P＜0.05).Conclusions Sig-1R ex-pression in rat brain tissue correlates with brain injury after cardiopulmonary resuscitation and potential mechanism seems to be neuronal protection through modulating mitochondrial function and endoplasmic reticulum stress.
