Knockdown of translocase of inner mitochondrial membrane 8A enhances gefitinib sensitivity to lung cancer cell line PC-9
10.16352/j.issn.1001-6325.2025.08.1073
- VernacularTitle:敲降线粒体内膜转运酶8A提升肺癌细胞系PC-9对吉非替尼治疗的敏感性
- Author:
Lei CHEN
1
;
Weihao REN
;
Lide WANG
Author Information
1. 北京大学首钢医院胸外科,北京 100144
- Keywords:
lung cancer;
targeted therapy;
gefitinib;
drug resistance;
TIMM8A
- From:
Basic & Clinical Medicine
2025;45(8):1073-1077
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the alteration and function of TIMM8A during gefitinib-induced growth inhibition of lung cancer cell line PC-9.Methods TIMM8A siRNA transfection experiment was used to inhibit the expression of TIMM8A in PC-9 and gefitinib resistant PC-9 cells(PC-9-MTAC).CCK-8 assay was carried out to assess PC-9 and PC-9-MTAC cell viability after gefitinib treatment or TIMM8A siRNA transfection.qPCR was used to determine TIMM8A expression in PC-9 or PC-9-MTAC cells.Results Gefitinib inhibited PC-9 cell growth in a dose-depend-ent manner.The expression of TIMM8 A was inhibited during this process,and more than 50％TIMM8 A expression had been inhibited by 25 nmol/L gefitinib,while knockdown of TIMM8 A enhanced inhibitory effects of gefitinib on PC-9 cell proliferation(P＜0.05).Compared with the sensitive cells,treatment of the gefitinib-resistant PC-9-MTAC with 100 nmol/L gefitinib for 48 h only inhibited approximately 30％of TIMM8A expression.Furthermore,inhibition of TIMM8A expression enhanced the sensitivity of PC-9-MTAC cells to gefitinib(P＜0.05).Conclusions Low expression of TIMM8A improves anti-tumor effects of gefitinib in lung cancer cell line PC-9.
