RPRD1B is highly expressed in human ovarian cancer cell lines and promotes tumor cell proliferation
10.16352/j.issn.1001-6325.2025.08.1066
- VernacularTitle:RPRD1B在人卵巢癌细胞系中高表达并促进肿瘤细胞增殖
- Author:
Ye TIAN
1
;
Quan HE
;
Xiaojuan WANG
Author Information
1. 清华大学北京清华长庚医院清华大学临床医学院,北京 102218;清华大学器官移植与仿生医学研究院,北京 102218
- Keywords:
regulation of nuclear pre-mRNA-domain-containing 1B(RPRD1B);
ovarian cancer;
proliferation
- From:
Basic & Clinical Medicine
2025;45(8):1066-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of regulation of nuclear pre-mRNA-domain-containing 1B(RPRD1B)in human ovarian cancer.Methods The expression level of RPRD1B in ovarian cancer was detected by RT-qPCR and Western blot experiments.Based on the CRISPR/Cas9 system and lentiviral system,the A2780 and SKOV3 cell lines with RPRD1B gene knockout and over-expression were respectively constructed,and their functions in ovarian cancer were verified by wound-healing assay,Transwell assay and mouse subcutaneous tumor formation model.Results RPRD1B was highly expressed in ovarian cancer cell lines(P＜0.001).Knockout of RPRD1B in-hibited the colony formation and proliferation ability of ovarian cancer cells(P＜0.001),as well as the cell migra-tion(P＜0.05)and invasion ability(P＜0.001).Meanwhile,knockout of RPRD1B inhibited the tumorigenesis ability of SKOV3 ovarian cancer cell lines in vivo(P＜0.001).Conclusions RPRD1B is highly expressed in hu-man ovarian cancer cell lines and promotes the growth of subcutaneous tumors in mice.
