Gypenoside inhibits the proliferation,migration and invasion of prostate cancer cell line PC-3
10.16352/j.issn.1001-6325.2025.08.1059
- VernacularTitle:绞股蓝皂苷抑制人前列腺癌细胞系PC-3增殖、迁移和侵袭
- Author:
Qingbo ZOU
1
;
Guochen ZHANG
;
Changjing PAN
Author Information
1. 济南市中西医结合医院泌尿外科,山东济南 271100
- Keywords:
gypenoside;
prostate cancer;
proliferation;
migration
- From:
Basic & Clinical Medicine
2025;45(8):1059-1065
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of gypenoside(Gyp)on proliferation,migration and invasion of hu-man prostate cancer cell line PC-3.Methods PC-3 cells were treated with 200-1 200 μg/mL Gyp to determine the optimal concentration.PC-3 cells were divided into control,Gyp-L group(400 μg/mL),Gyp-M group(600 μg/mL)and Gyp-H group(800 μg/mL),and Gyp-H+8-bromo-cAMP group(100 μmol/L PKA activator).Colony formation assay was applied to detect cell proliferation.Apoptosis was measured by flow cytometry.Scratch experiment was applied to detect the migration ability.Transwell assay was applied to detect the invasive ability.ELISA experiment was applied to detect cAMP level in each group.Western blot was applied to detect the expres-sion of E-cadherin,N-cadherin,Snail,PKA and CREB proteins.Results Gyp inhibited PC-3 proliferation in a concentration dependent manner.Gyp concentration of 400,600 and 800 μg/mL was selected for subsequent experiments.Compared with the control group,colony formation,number of invasive cells,scratch healing rate,the levels of Snail,N-cadherin,PKA,cAMP,and CREB proteins in the Gyp-L,Gyp-M,and Gyp-H groups were significantly reduced(P＜0.05),but the apoptosis rate as well as the level of E-cadherin protein were significantly increased(P＜0.05).PKA activator attenuated the inhibitory effect of Gyp on the malignant behavior of prostate cancer cells.Conclusions Gyp inhibits proliferation,migration and invasion,and promotes apoptosis in prostate cancer cell line PC-3.
