Scutellarin inhibits proliferation and migration of human gastric cancer cell line MGC803
10.16352/j.issn.1001-6325.2025.08.1048
- VernacularTitle:灯盏花乙素抑制人胃癌细胞系MGC803增殖和迁移
- Author:
Chunliang JIA
1
;
Lei LIANG
;
Hansong LI
;
Jian WANG
;
Lei ZHANG
;
Qingke LI
;
Yuan YAO
Author Information
1. 唐山市人民医院胃肠外一科,河北唐山 063000
- Keywords:
scutellarin;
nuclear factor kappa-B signaling pathway;
gastric cancer
- From:
Basic & Clinical Medicine
2025;45(8):1048-1053
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of scutellarin(SCU)through regulation of nuclear transcription factor kappa B(NF-κB)p65 signaling pathway on the proliferation and migration of gastric cancer cells line MGC803.Methods To select SCUs with concentrations of 100,250,and 500 nmol/L for subsequent experiments;MGC803 gastric cancer cells were separated into a control group,SCU-L group(100 nmol/L),SCU-M group(250 nmol/L),SCU-H group(500 nmol/L),SCU-H+PMA group(NF-κB activator,200 nmol/L PMA).Cell plate cloning experiments were applied to detect cell proliferation.Scratch healing experiment was used to detect the migration of MGC803 cells;Tran swell assay was used to detect the invasive ability of MGC803 cells;Enzyme linked immuno-sorbent assay(ELISA)was used to detect the level of IFN-γ,IL-4,and IL-10 in the supernatant of MGC803 cells;Western blot detection of NF-κB p65 pathway related protein expression.Results SCU inhibited proliferation of MGC803 in a concentration dependent manner.SCU concentrations of 100,250,and 500 nmol/L were selected for subsequent experiments.Compared with the control group,the number of cell clones formed,scratch healing rate,number of invasive cells,IL-10 level and p-p65/p65 ratio all decreased in SCU-L group,SCU-M group,and SCU-H group,while IFN-γ and IL-4 increased(P＜0.05).Compared with the SCU-H group,the number of cell clones,scratch healing rate,number of in-vasive cells,IL-10 level,and p-p65/p65 ratio obviously increased in the SCU-H+PMA group,while IFN-γ and IL-4 level significantly reduced(P＜0.05).Conclusions SCU may inhibit the proliferation and migration of gastric cancer cell line MGC803.
