Correlation of serum miR-185-3p and miR-3194-5p with the severity of coronary artery lesions
10.16352/j.issn.1001-6325.2025.08.1041
- VernacularTitle:血清miR-185-3p和miR-3194-5p水平与冠状动脉病变严重程度的相关性
- Author:
Yanxun CHEN
1
;
Feilong ZHAO
;
Jing YANG
Author Information
1. 河南科技大学第一附属医院心血管内科一病区,河南郑州 471000
- Keywords:
coronary heart disease;
miR-185-3p;
miR-3194-5p;
severity of coronary artery disease
- From:
Basic & Clinical Medicine
2025;45(8):1041-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation of serum miR-185-3p and miR-3194-5p with lesion severity of coronary artery.Methods A total of 88 patients with coronary artery disease,diagnosed and treated at the First Affil-iated Hospital of Henan University of Science and Technology between January 2021 and July 2024,were enrolled as the research group.This group comprised 40 patients in the low-score subgroup and 48 in the high-score subgroup.The control group consisted of 36 healthy individuals undergoing physical examinations during the same period.The level of miR-185-3p and miR-3194-5p in serum was detected using real-time fluorescence quantitative PCR(RT-qPCR)method.Pearson correlation analysis was applied to assess the correlation between serum miR-185-3p and miR-3194-5p levels in the research group.The correlation between the levels of miR-185-3p and miR-3194-5p with the severity of coronary artery disease were analyzed using Spearman correlation analysis.Logistic regression was ap-plied to analyze the influencing factors of high-score coronary heart disease lesions.ROC curve was applied to analyze the diagnostic value of serum miR-185-3p and miR-3194-5p levels for high-score lesions in clinical coronary heart dis-ease.Results The serum miR-185-3p level in the control group was significantly lower than that in the research group,and the miR-185-3p level in the low-score group was lower than that in the high-score group(P＜0.05).The miR-3194-5p level in the research group was significantly lower than that in the control group,and the miR-3194-5p level in the high-score group was lower than that in the low-score group(P＜0.05).Serum miR-185-3p and miR-3194-5p in the research group were negatively correlated(P＜0.05),and both were correlated with the severity of coronary artery disease(P＜0.05).Logistic regression analysis showed that the history of diabetes,hypertension,apolipoprotein B,fasting blood glucose,high-sensitivity C-reactive protein,serum miR-185-3p and miR-3194-5p were all the influencing factors for the occurrence of coronary heart disease with high score(P＜0.05).The area under the curve(AUC)for the combined diagnosis of miR-185-3p and miR-3194-5p in high-score coronary heart disease lesions was significantly higher than that for their individual diagnosis(P＜0.05).Conclusions The serum miR-185-3p level in the research group is significantly higher than that in the control group,and the miR-3194-5p level is lower than the control group.The serum miR-185-3p level is positively correlated with the severity of coronary artery disease,while the serum miR-3194-5p level is negatively correlated with the severity of coronary artery disease.The combined detection of the two is of great significance for the clinical diagnosis of high-score lesions in coronary heart disease.
