Effect of body weight and length on carotid artery vascular stenosis induced by balloon strain in rats
10.16352/j.issn.1001-6325.2025.08.1034
- VernacularTitle:大鼠体质量和体长对颈总动脉球囊拉伤致血管狭窄模型的影响
- Author:
Tianwang CHEN
1
;
Jiawei LUO
;
Yidan YUAN
;
Yimin YANG
;
Bo HUANG
;
Shangfu XU
;
Lisheng LI
Author Information
1. 遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,贵州遵义 563000;遵义医科大学贵州省基础药理重点实验室药学院,贵州遵义 563000
- Keywords:
restenosis;
angioplasty;
balloon catheter;
rat
- From:
Basic & Clinical Medicine
2025;45(8):1034-1040
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of body weight and length of SD rats on the carotid artery balloon injury-induced vascular stenosis model in order to provide a reference for replicating an ideal vascular stenosis mod-el.Methods Male rats were divided into three groups based on body weight and length.The CONQUEROR? SC PTCA balloon catheter was employed,with a fixed balloon inflation volume of 0.2 mL to induce injury in the left common carotid artery,while the right side served as a control.As soon as surgery operation,one rat from each group was selected for Evans Blue dye verification.Fourteen days later,the injured and contra lateral common ca-rotid arteries from remaining rats were harvested for HE staining to check the extent of stenosis.Based on these find-ings,six rats within the optimal range of body weight and length were selected for further validation.Results Rats with body weights ranging from 280 to 380 g(corresponding body lengths of 21.0-26.5 cm)underwent balloon catheter injury,resulting in endothelial detachment and varying degrees of stenosis in the common carotid artery.In rats weighing 280-300 g(body lengths of 21.0-22.5 cm)had severe stenosis or occlusion of the common carotid artery with thrombosis.In rats weighing 320-340 g(body lengths of 23.0-24.5 cm),the internal and external elastic plates of the common carotid artery were ruptured and the vascular morphology was abnormal.Conversely,rats weighing 360-380 g(body lengths of 25.0-26.5 cm)did not show any ruptured elastic laminae or thrombus formation in the common carotid artery,and the extent of vascular stenos in rats with a body weight of 360 g was moderate and uniform.The results of the repeated validation experiments were consistent.Conclusions Rats with a body weight range of 360 g(corresponding body length of 25.0-26.5 cm)are suitable for development of an ideal vascular stenosis model.
