Propofol alleviates neuropathic pain induced by spinal nerve ligation in rats
10.16352/j.issn.1001-6325.2025.08.1028
- VernacularTitle:异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛
- Author:
Yandong GAO
1
;
Burong BIAN
;
Bo WANG
Author Information
1. 榆林市第一医院(延安大学第二附属医院)手术麻醉科,陕西榆林 719000
- Keywords:
propofol;
spinal nerve ligation;
neuropathic pain;
NO/cGMP signaling pathway
- From:
Basic & Clinical Medicine
2025;45(8):1028-1033
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the improvement effect and mechanism of propofol on neuropathic pain(NP)model rats induced by spinal nerve ligation(SNL).Methods An SNL rat model was established,and 30 success-fully modeled rats were randomly divided into SNL group,propofol+SNL group and propofol+7-N1(7-nitroindazole)+SNL group(n=10 each),with sham group as control(n=10).After propofol intervention,Von Frey method was applied to detect mechanical hypersensitivity in rats.The hot claw foot analgesic device was applied to detect the la-tent period of the heat contraction foot reflex.Enzyme-linked immunosorbent assay(ELISA)method was applied to detect the expression level of interleukin-6(IL-6)and interferon-γ(IFN-γ)in the spinal cord of rats.RT-qPCR method was used to detect the mRNA expressions of suppressor of cytokine signaling 3(SOCS3),arginase-1(Arg-1)and cluster of differentiation 68(CD68)in the spinal dorsal horn.Western blot was applied to detect the expression level of proteins related to the nitric oxide/cyclic guanosine monophosphate(NO/cGMP)signaling pathway.Results Compared with the sham group,the mechanical foot contraction reflex threshold,thermal foot contraction reflex latency,and the expression level of Arg-1 mRNA,endothelial nitric oxide synthase(eNOS)and soluble gua-nylate cyclase(sGCa)proteins were significantly reduced in the SNL group(P＜0.05),the level of IL-6 and IFN-γ and the expression level of SOCS3,CD68 mRNA and inducible nitric oxide synthase(iNOS)protein were signifi-cantly increased(P＜0.05).Compared with the SNL group,the mechanical foot reflex threshold,thermal foot reflex latency of rats in the propofol+SNL group,the expression level of Arg-1 mRNA,eNOS and sGCa protein were sig-nificantly increased(P＜0.05),the level of IL-6 and IFN-γ and the expression of SOCS3,CD68 mRNA and iNOS protein were all decreased(P＜0.05).The NO/cGMP signaling pathway inhibitor 7-N1 reversed the improvement effect of propofol on SNL rats(P＜0.05).Conclusions Propofol alleviates NP and injury in SNL rats by activating the NO/cGMP signaling pathway.
