Construction of Hsp90-based fluorescent molecular probe and evaluation of pancreatic tumor recognition effects
10.16352/j.issn.1001-6325.2025.07.0905
- VernacularTitle:基于Hsp90的荧光分子探针的构建与胰腺肿瘤识别效应评价
- Author:
Haojun LUO
1
;
Dexin KONG
;
Wei HUANG
;
Nan YANG
;
Yanyong LIU
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 药理学系,北京 100005
- Keywords:
heat shock protein 90(Hsp90);
tumor recognition;
peptide
- From:
Basic & Clinical Medicine
2025;45(7):905-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct fluorescent molecular probes targeting at tumor heat shock protein 90(Hsp90)in order to enhance tumor-specific recognition.Methods The human pancreatic adenocarcinoma cell line PANC-1 and the human small cell lung cancer cell line NCI-H446 were used as the research targets to study the uptake and clearance of Cy5-P7 by tumor cells with flow cytometry as well as immune-fluorescence technology;The mechanism to mediate entrance of Cy5-P7 into the cells by Hsp90 was investigated by blocking of the PANC-1 cell surface pro-teins with monoclonal antibody to Hsp90;Human pancreatic cancer cell line PANC-1 was subcutaneously injected into posterior dorsum of BALB/c nude mice to construct a xenogeneic subcutaneous tumor model to validate the in vivo tumor recognition ability by Cy7-P7 as well as its in vivo distribution in mice.Results The uptake of Cy5-P7 by cells was significantly reduced in low temperature(P<0.05);An average fluorescence intensity in Cy5-P7-trea-ted cells was significantly reduced after blocking with a monoclonal antibody to Hsp90(P<0.05);The fluorescence intensity at the tumor site of Cy7-P7 group was higher than that of control group(P<0.05),and there was a significant presence of Cy7-P7 in the kidney and tumor fluorescence.Conclusions Cy5-P7 can effectively target at Hsp90,and its specific binding significantly enhanced the cellular uptake of Cy5-P7 fluorescent probe,which im-proved the sensitivity and accuracy of fluorescence imaging.Cy7-P7 showed good tumor active recognition in vivo,and was able to be enriched at the tumor site and metabolized out of the body in 48 h,which may effectively sup-port accurate tumor recognition.
