Angiopoietin-like protein 8 promotes apoptosis of vascular smooth muscle cells through activating p53 signaling pathway
10.16352/j.issn.1001-6325.2025.07.0882
- VernacularTitle:血管生成素样蛋白8通过激活p53信号通路促进平滑肌细胞凋亡
- Author:
Huahui YU
1
;
Zhiyong DU
;
Xiaolu JIAO
;
Qianwen LYU
;
Yunyun YANG
;
Lijie HAN
;
Yanwen QIN
Author Information
1. 首都医科大学附属北京安贞医院 北京市心肺血管疾病研究所,北京 100029
- Keywords:
angiopoietin-like proteins 8;
vascular smooth muscle cells;
apoptosis;
p53 pathway
- From:
Basic & Clinical Medicine
2025;45(7):882-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which angiopoietin-like protein 8(ANGPTL8)regulates vascular smooth muscle cell(VSMCs)apoptosis.Methods An in vitro abdominal aortic aneurysm cell model was established by stimulating human VSMCs(HUSMCs)with angiotensin Ⅱ(AngⅡ).Stable ANGPTL8 knockdown and over-expression VSMC cell strains were generated using lentiviral transfection.TUNEL staining was used to de-tect apoptosis.Western blot analysis was performed to measure the protein expression of ANGPTL8,caspase9,caspase3,Bcl-2,Bax,p53,and PUMA,while RT-qPCR was used to assess mRNA expression of ANGPTL8,Bcl-2 and Bax.Results AngⅡ significantly induced ANGPTL8 expression in HVSMCs in a time-and dose-de-pendent manner(P＜0.05).ANGPTL8 knockdown significantly reduced the expression of apoptosis-related proteins caspase9,caspase3,and Bax,while increased the expression of the anti-apoptotic protein Bcl-2(P＜0.05).Con-versely,ANGPTL8 over-expression markedly induced HVSMCs apoptosis,which was significantly suppressed by treatment with the p53 pathway inhibitor pifithrin-α(PFT-α).Conclusions ANGPTL8 may promote VSMC apop-tosis by activation of p53 signaling pathway.
