HJT-sRNA-m7 bencaosome modulates fibrosis progression in a silicosis mouse model
10.16352/j.issn.1001-6325.2025.07.0874
- VernacularTitle:HJT-sRNA-m7本草体控制硅肺模型小鼠的纤维化进程
- Author:
Jiahui CHANG
1
;
Pengju REN
;
Yunyi ZHOU
;
Chengyu JIANG
;
Yanli ZHANG
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系重大疾病共性机制研究全国重点实验室,北京 100005
- Keywords:
silicosis;
pulmonary fibrosis;
oligonucleotide therapy;
immune regulation
- From:
Basic & Clinical Medicine
2025;45(7):874-881
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and mechanism of HJT-sRNA-m7(M7)bencaosome in a silicosis mouse model.Methods C57BL/6J mice were randomly divided into four groups:blank,control,negative control(NC)oligonucleotide,and M7 treatment(HJT-sRNA-m7 bencaosome)groups.After three rounds of pretreatment with HJT-sRNA-m7 bencaosome,all groups except the blank one were modeled via a single intratracheal exposure.Each mouse received 50 μL of a silica suspension at a dose of 200 mg/kg body weight via intratracheal instillation.From day 6 to day 26,the bencaosome was administered every other day via oral gavages.On day 28,pulmonary function tests were performed.Bronchoalveolar lavage fluid was collected for flow cytometry and cytokine analysis.The left lung was harvested for histopathological examination and Masson's trichrome staining to evaluate collagen fiber dep-osition.The right lung was used for hydroxyproline quantification to assess collagen accumulation.Results The re-sults of pulmonary function test,pathological analysis and hydroxyproline measurements all indicated that M7 ben-caosome treatment significantly alleviated silica-induced pulmonary fibrosis.Moreover,flow cytometry analysis of BALF confirmed that M7 bencaosome inhibited the silica-induced inflammatory response,that was supported by cy-tokine analysis.Conclusions HJT-sRNA-m7 bencaosome is quite effective to treat silicosis and inhibits mitigating pulmonary fibrosis progression in mouse models.
