Study of dynamic transcriptome during mitosis of HAP1 cells
10.16352/j.issn.1001-6325.2025.06.0786
- VernacularTitle:HAP1细胞有丝分裂过程中的动态转录物组研究
- Author:
Xueli WU
1
;
Yurong YANG
;
Yongli BAO
;
Rucheng WU
;
Lisha WANG
;
Yang CHEN
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系重大疾病共性机制研究全国重点实验室,北京 100005
- Keywords:
mitosis;
transcriptome;
cell cycle synchronization;
signaling pathway regulation
- From:
Basic & Clinical Medicine
2025;45(6):786-792
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comprehensively understand the map of transcripts during mitosis and their regulatory mechanisms of HAP 1 cells by conducting transcriptome sequencing analysis after being released by mitotic synchro-nization arrest.Methods HAP1 cells were subjected to mitotic synchronous arrest with nocodazole and samples were collected after 0,20,80 min release,and RNA sequencing(RNA-seq)were performed.The transcriptome data was cleaned and the differentially expressed genes,expression trend clustering and functional enrichment com-bined with the protein interaction network were analyzed to explore the changes of signaling pathways in HAP 1 cells during mitosis.Results The transcriptome of HAP1 cells after synchronous release from mitosis underwent significant changes in time series,and differential gene cluster analysis revealed four gene clusters were enriched in important biological processes such as p53 signaling and cytoplasmic translation.Conclusions The transcriptome time-dependent dynamic changes during mitosis in HAP1 cells are coordinated regulation of key signaling pathways including cellular stress response,translational control and chromatin remodeling,ensuring a balance between growth and stress response upon mitotic exit.
