Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87
10.16352/j.issn.1001-6325.2025.06.0777
- VernacularTitle:MMSN@Gem的构建及其抑制人膀胱癌细胞系BIU-87增殖和促凋亡
- Author:
Daya WANG
1
;
Zhijia LI
;
Dewei ZHAO
;
Ximeng CHEN
Author Information
1. 温州市中心医院泌尿外科,浙江温州 325000
- Keywords:
gemcitabine;
nano co-loading system;
bladder tumour;
mesoporous silica
- From:
Basic & Clinical Medicine
2025;45(6):777-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a multifunctional mesoporous silica-based nanocarrier system(MMSN@Gem)with gemcitabine and investigate its effect on bladder cancer cells BIU-87.Methods The multifunctional meso-porous silica-based nano drug-carrying system was prepared by a modified method.Transmission electron microsco-py,high-performance liquid chromatography,and thermal imaging were used to characterize the morphology,drug-carrying and photothermal properties of MMSN@Gem.The effect of MMSN@Gem on BIU-87 bladder cancer cells was detected by in vitro experiments.Results MMSN@Gem exhibited a well-defined spherical morphology with an average particle size of(102.48±1.03)nm with a drug loading capacity of 25.04％±0.17％,and an average zeta potential of(-24.84±0.07)mV.The photothermal conversion efficiency was 40.7％which significantly enhanced the release of Gem under near-infrared irradiation.In vitro studies showed that MMSN@Gem significantly inhibited BIU-87 cell activity,induced apoptosis of BIU-87 cells,reduced migration and invasion ability,and enhanced its uptake by BIU-87 cells.Conclusions MMSN@Gem exhibits excellent photothermal properties,enhances cellular uptake efficiency,inhibits the proliferation and migration of BIU-87 cells,and promotes apoptosis,providing a promising drug delivery system for the clinical treatment of bladder cancer.
