Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models
10.16352/j.issn.1001-6325.2025.06.0770
- VernacularTitle:内皮细胞增殖及细胞外基质相关通路调控模型大鼠动脉性肺动脉高压
- Author:
Xiaoxuan LU
1
;
Lina GUO
;
Hong ZHANG
;
Wanlu SONG
;
Yufei HU
;
Peiran YANG
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院生理学系,北京 100005
- Keywords:
pulmonary artery hypertension(PAH);
animal model;
endothelial cell;
extracellular matrix(ECM)
- From:
Basic & Clinical Medicine
2025;45(6):770-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify mechanisms regulating disease progression in rat models of pulmonary arterial hy-pertension(PAH).Methods Rat PAH models were established using subcutaneous monocrotaline(MCT)injec-tion and the SU5416/hypoxia(SU/Hx)method.Transcriptomic sequencing of lung tissues was performed to identify gene expression and pathway alterations in PAH rats,followed by a comparative analysis with transcriptomic data of patients with idiopathic pulmonary arterial hypertension(IPAH)in NCBI database.Results Inflammatory-related genes such as CXCL9,CCL24,and SECTM1 were upregulated in both PAH rat models and IPAH patient lungs,while genes such as DGKG and DOCK9 were downregulated(P＜0.05).Pathways related to endothelial cell proliferation regulation and extracellular matrix(ECM)remodeling were significantly upregulated(P＜0.05).Conclusions The imbalance in endothelial cell proliferation and abnormal ECM remodeling may collectively contribute to PAH pathogenesis.Further exploration of these signaling pathways may provide deep in-sights for early diagnosis and targeted therapy of PAH.
