Inhibitory effect of β-elemonic acid on proliferation and invasion of colon cancer cell lines
10.16352/j.issn.1001-6325.2025.06.0748
- VernacularTitle:β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用
- Author:
Ting SUN
1
;
Teng ZOU
;
Yisong YANG
;
Shuangping LIU
;
Xin REN
;
Dan WANG
Author Information
1. 大连大学医学院检验系,辽宁大连 116622
- Keywords:
β-elemonic acid;
colon cancer;
apoptosis;
MMP2;
PI3K/Akt/mTOR pathway
- From:
Basic & Clinical Medicine
2025;45(6):748-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of β-elemonic acid(β-EA)on the proliferation and inva-sion of colon cancer cells and the underlying mechanisms through PI3K/AKT/mTOR signaling pathway.Methods The effects of β-EA on colon cancer cell proliferation were evaluated using the MTT assay and colony formation as-say.Transwell invasion assay were used to assess the impact of β-EA on invasion.Western blot analysis was con-ducted to detect changes in PI3K/AKT/mTOR pathway proteins after treatment.Results MTT assay showed thatβ-EA effectively inhibited the proliferation of colon cancer HCT8 and HCT116 cells in a dose-dependent manner.The colony formation assay confirmed its inhibitory effect on cell proliferation.Transwell invasion assays demonstra-ted that β-elemonic acid reduced the invasion abilities of the cells.Western blot analysis revealed increased expression of apoptosis-related proteins cleaved-caspase 3,cleaved-caspase 9,and Bax,while Bcl-2 expression was decreased.Invasion-related proteins vimentin,snail,MMP2,and MMP9 were downregulated after treatment.Addi-tionally,β-EAA reduced the levels of p-PI3K,p-Akt,and p-mTOR,and these reductions were more pronounced af-ter the addition of the PI3K inhibitor LY294002.Conclusions β-EA may inhibit proliferation and invasion in colon cancer cell lines HCT8 and HCT116 through PI3K/AKT/mTOR signaling pathway,and potentially be transformed as a novel therapeutic agent for colon cancer.
