Pseudogene GTF3AP2 is involved in the regulation of erythroid differentiation
10.16352/j.issn.1001-6325.2025.06.0735
- VernacularTitle:假基因GTF3AP2参与调控造血干祖细胞向红系分化
- Author:
Lan XIA
1
;
Yu RONG
;
Hongkai XIA
;
Yanni MA
;
Jia YU
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系重大疾病共性机制研究全国重点实验室,北京 100005
- Keywords:
pseudogene;
erythroid differentiation;
transcriptome sequencing;
GTF3AP2
- From:
Basic & Clinical Medicine
2025;45(6):735-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pseudogene GTF3AP2 in erythroid differentiation.Methods The published high-throughput RNA sequencing(RNA-seq)data were analyzed to identify the functional pseudogene GTF3AP2,which may play a role in erythropoiesis.The endogenous expression of GTF3AP2 was inhibited by shR-NA in CD34+hematopoietic stem/progenitor cells to assess the colony-forming ability through colony-forming assay.Flow cytometry analysis was applied to detect changes in the ratio of erythroid/megakaryocytic progenitor cells.Ad-ditionally,the role of GTF3AP2 in erythroid differentiation was determined through transcriptome sequencing,which revealed alterations at the cellular and molecular levels following the knockdown of GTF3AP2.Results Com-pared with the sh-EV group,knockdown of GTF3AP2 resulted in a significant increase in cell expansion,character-ized by a significant rise in the number of colony-forming unit erythroid cells(P＜0.001),an increase in the proportion of CD71+CD235a+erythroid precursors(P＜0.01),and a decrease in the proportion of CD71-CD235a+mature erythrocytes(P＜0.05).Furthermore,there was a significant reduction in the expression of key erythroid dif-ferentiation genes,including KLF1,HBB,GYPA,EPOR and TFRC.Conclusions Knocking down of GTF3AP2 promotes the expansion of erythroid precursor cells and inhibits erythroid maturation,suggesting that GTF3AP2 plays a regulatory role in erythroid differentiation.
