Analysis of key immune-related genes and drug candidates in globoid cell leukodystrophy
10.16352/j.issn.1001-6325.2025.06.0727
- VernacularTitle:球形细胞脑白质营养不良的关键免疫相关基因和药物候选物分析
- Author:
Guiyuan LUO
1
;
Yang LI
;
Ye ZHANG
;
Yafeng LYU
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系,北京 100005
- Keywords:
globoid cell leukodystrophy;
Krabbe disease;
neural stem cells;
immune-related gene
- From:
Basic & Clinical Medicine
2025;45(6):727-734
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the key immune-related genes and potential drug targets of Krabbe disease by using neural stem cells(NSCs)derived from induced pluripotent stem cells(iPSCs)generated from patients with Krabbe disease.Methods 1)The transcriptome data of GLD-NSCs(GSE212512)was analyzed using GEO2R and Sangerbox;2)Differential expression genes(DEGs)related to immunity were identified through various methods;3)Functional enrichment and pathway analysis were performed using DAVID;4)The protein-protein interaction network was constructed and analyzed using STRING and Cytoscape;5)Key immune-related genes were deter-mined through topological analysis;6)Potential therapeutic drugs were screened using DGIdb and drug MAP data-bases;7)Molecular docking was performed using CB-DOCK2.Results 1)Sixty-one immune-related DEGs were identified in patient NSCs,with GDNF,EGF,KDR,FGF10,and MET being identified as key immune-related genes;2)Drug candidates with high binding affinity to these genes were identified,including gentamicin for GDNF,cetuximab for EGF,tivozanib for KDR,and capmatinib for MET.Conclusions The predictions suggest cetuximab,gentamicin,tivozanib,and capmatinib exhibit high binding affinity with EGF,GDNF,KDR,and MET,respectively,indicating their potential application value in GLD targeted therapy.
