Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice
10.16352/j.issn.1001-6325.2025.06.0714
- VernacularTitle:Snhg3通过促进Sestrin2的表达改善小鼠糖代谢
- Author:
Minglong ZHANG
1
;
Mingyue GAO
;
Xianghong XIE
;
Zeyu GUO
;
Xiaojun LIU
;
Li YAN
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院病理生理学系,北京 100005;中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系,北京 100005
- Keywords:
small nucleolar RNA host gene 3(Snhg3);
hepatic gluconeogenesis;
stress-inducing protein 2(Sestrin 2)
- From:
Basic & Clinical Medicine
2025;45(6):714-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3)and its regulatory mechanism in the hepatic glucose metabolism of mice.Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice,and then glucose tolerance and pyruvate tolerance were meas-ured.The mRNA expression of mouse liver gluconeogenesis-related genes phosphoenolpyruvate carboxylase(Pepck)and glucose-6-phosphatase(G6pc)and stress-inducing protein 2(Sestrin2,Sesn2,a gene adjacent to Snhg3)were de-tected by RT-qPCR.The dual luciferase reporter assay was used to detect the effect of Snhg3 on the Sesn2 promoter activity in 293T cells.Results Snhg3 over-expression improved glucose tolerance and pyruvate tolerance in db/db mice.Snhg3 over-expression inhibited the mRNA of gluconeogenesis genes of Pepck(P＜0.05)and G6pc(P＜0.05),while promoted the mRNA of Sesn2(P＜0.01).Meanwhile,Snhg3 over-expression promoted Sesn2 promoter activity in 293T cells(P＜0.05).Conclusions Snhg3 improves glucose metabolism in mice by promoting Sestrin2 expression.
