GOT2 regulates aspartate levels and viability in acute myeloid leukemia cells
10.16352/j.issn.1001-6325.2025.05.0622
- VernacularTitle:GOT2调控急性髓系白血病细胞的天冬氨酸含量和存活
- Author:
Yiyang LIU
1
;
Fang WANG
;
Jia YU
;
Weiqian LI
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 医学分子生物学国家重点实验室,北京 100005
- Keywords:
GOT2;
acute myeloid leukemia;
aspartate
- From:
Basic & Clinical Medicine
2025;45(5):622-626
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of the glutamic-oxaloacetic transaminase 2(GOT2)gene in acute myeloid leukemia cells.Methods ShRNAs targeting at GOT2 were constructed to suppress GOT2 expression in the THP-1 human monocytic leukemia cell line.GOT2 mRNA level was measured by RT-qPCR,and GOT2 protein ex-pression was measured by Western blot.Intracellular free amino acid level was quantified using colorimetric assays.The impact of GOT2 knockdown in THP-1 cell function was evaluated through cell viability(CCK-8 assay)and ap-optosis(flow cytometry).Results After GOT2 knockdown,both GOT2 mRNA and protein were significantly de-creased in THP-1 cells(P＜0.05).Compared to the control group,GOT2 knockdown did not affect intracellular glutamate levels(P＞0.05),but led to a marked decrease in aspartate level(P＜0.05).GOT2 knockdown signifi-cantly impaired THP-1 cell viability,inhibited cell proliferation(P＜0.05)and promoted apoptosis(P＜0.05).Conclusions Down regulation of GOT2 significantly decreases intracellular aspartate level in acute myeloid leukemia cells,impairs cellular viability and induces apoptosis,which suggests that GOT2 may play a key role in the regula-tion of amino acid metabolism in acute myeloid leukemia.
