Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice
10.16352/j.issn.1001-6325.2025.05.0616
- VernacularTitle:敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平
- Author:
Yuhan LIU
1
;
Yingying WANG
;
Yucheng WANG
;
Lei GUO
;
Rui JU
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院药理系,北京 100005
- Keywords:
macrophages;
sepsis;
Mcart1;
inflammation
- From:
Basic & Clinical Medicine
2025;45(5):616-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Mcart1 knockout on acute inflammation of macrophages in vitro and in vivo.Methods The Mcart1 knockout mice were used to establish a sepsis model induced by lipopolysaccharide(LPS),and the survival period was measured.Bone marrow derived macrophages(BMDM)of LPS-induced inflam-mation mice were cultured in DMEM high-glucose medium.The mRNA levels of M1 and M2 related cytokines of BM-DM after LPS stimulation were detected by RT-qPCR.The expression level of inflammation-related cytokines in serum of sepsis mice was detected by ELISA.Results Compared with wild type mice(Mcart1flox/flox),the survival time length of sepsis in Mcart1 knockout mice(Mcart1Lyz2-Cre)was significantly shortened(P＜0.001).After inflammation,the mRNA level of M1-related cytokines was up-regulated in BMDM cells of Mcart1Lyz2-Cre mice(P＜0.05);The mRNA level of M1-related cytokines was down-regulated(P＜0.05);The expression of M1-related mediators in serum of sep-sis Mcart1Lyz2-Cre mice was up-regulated(P＜0.01).Conclusions Mcart1 knockout can significantly raise the inflam-matory response of macrophages and aggravate the pathological symptoms of sepsis mice.
