TOMM40L promotes proliferation and migration of triple negative breast cancer cells and correlates with poor prognosis
10.16352/j.issn.1001-6325.2025.05.0575
- VernacularTitle:TOMM40L促进三阴性乳腺癌细胞增殖和迁移并与不良预后相关
- Author:
Ke ZHANG
1
;
Jiangning LU
;
Lixin SUN
;
Long YU
;
Lichao SUN
;
Yuliang RAN
Author Information
1. 国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院 北京协和医学院 肿瘤医院 分子肿瘤学国家重点实验室,北京 100021
- Keywords:
triple-negative breast cancer;
TOMM40L;
clinical significance
- From:
Basic & Clinical Medicine
2025;45(5):575-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance and to investigate the expression of TOMM40L in the tis-sue of triple-negative breast cancer(TNBC).Methods The expression of TOMM40L in TNBC tissues and normal tissues was analyzed with TCGA and UALCAN databases.Univariate and multivariate Cox regression analysis and Nomogram model were used to evaluate the prognostic value of TOMM40L in TNBC patients.Furthermore,the ex-pression of TOMM40L in breast cancer cell lines was evaluated using Western blot analysis and quantitative real-time PCR.Specific siRNA knockdown was performed to evaluate the migration,and cell proliferation of TOMM40L.The potential signaling pathways of TOMM40L were identified by GO and KEGG and GSEA.Results TOMM40L was highly expressed in the TNBC compared to non-TNBC tumor tissues(P＜0.001).TOMM40L levels were en-hanced in TNBC cell lines as compared to other non-TNBC cell lines.CCK-8 and Transwell assay demonstrated that TOMM40L knockdown reduced the proliferation and migration of TNBCcell lines.Functional enrichment analysis showed that TOMM40L was involved in glucose metabolism-related pathways.Conclusions The expression of TOMM40L is increased in TNBC and is correlated with poor prognosis.TOMM40L may promote TNBC migration and proliferation.
