Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells

VernacularTitle:假基因AC106872.1参与维持人类胚胎干细胞自我更新能力
Author: Zhengyang JIANG ¹ ; Mengyao SUN ; Liu HE ; Jia YU ; Yanni MA
Author Information

1. 中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系,北京 100005
Keywords: pseudogene; human embryonic stem cells; pluripotency
From: Basic & Clinical Medicine 2025;45(5):561-567
CountryChina
Language:Chinese
Abstract: Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells(hESCs).Methods AC106872.1 was knocked out in hESCs and knockout effi-ciency was validated by PCR and agarose gel electrophoresis.The colony formation of hESCs was assessed through colony formation assays and alkaline phosphatase(AP)staining.The expression level of pluripotency and differ-entiation marker genes was analyzed by qPCR and flow cytometry.RNA sequencing(RNA-seq)was performed to assess transcriptomic changes upon AC106872.1 knockout.Results Knockout of AC106872.1 significantly in-hibited the colony formation of hESCs(P＜0.05).The expression level of pluripotency marker genes was signifi-cantly reduced(P＜0.000 1),while the expression of differentiation marker genes was markedly increased(P＜0.000 1).Conclusions The pseudogene AC 1 06872.1 plays a crucial role in maintaining human embryonic stem cell self-renewal through regulation of pluripotency genes expression.