Upregulation of miR-152 inhibits proliferation and migration of human vascular smooth muscle cells induced by ox-LDL
10.16352/j.issn.1001-6325.2025.04.0486
- VernacularTitle:上调miR-152抑制ox-LDL诱导的人血管平滑肌细胞增殖和迁移
- Author:
Jing ZHOU
1
;
Miao QU
Author Information
1. 宝鸡市中心医院功能科,陕西宝鸡 721008
- Keywords:
miR-152;
atherosclerosis;
Rho-associated coiled-coil kinase 1;
vascular smooth muscle;
proliferation;
migration
- From:
Basic & Clinical Medicine
2025;45(4):486-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of miR-152 and Rho-associated coiled-coil kinase 1(ROCK1)in vas-cular smooth muscle cells(VSMCs)treated with oxidized low-density lipoprotein(ox-LDL).Methods Human vascu-lar smooth muscle cells CRL-1999 were intervened with ox-LDL to establish an in vitro atherosclerosis model.miR-152 mimic and ROCK1 siRNA were transfected into corresponding cells.RT-qPCR was used to detect the expression of miR-152 and ROCK1 mRNA.The CCK-8 method was used to assess cell proliferation activity,and the Transwell as-say was used to test cell migration ability.A luciferase reporter gene assay was conducted to determine the targeting relationship between miR-152 and ROCK1.Results CRL-1999 cells treated with 100 μg/mL ox-LDL for 48 hours showed the maximum proliferation rate.Compared with the untreated control group,the relative expression of miR-152 in the ox-LDL-induced group was significantly decreased(P＜0.05).Transfection of miR-152 mimic significantly re-duced the proliferation and migration of CRL-1999 cells(P＜0.05).The luciferase reporter gene assay indicated that miR-152 targets ROCK1,and expression of ROCK1 mRNA and protein in miR-152 mimic group was reduced(P＜0.05).Transfection of ROCK1 siRNA also significantly decreased the proliferation and migration of CRL-1999 cells(P＜0.05).Conclusions miR-152 inhibits the ox-LDL induced proliferation and migration of CRL-1999 cells by downregulating ROCK1 expression,which may be a potential therapeutic target for atherosclerosis.
