Evaluation of chemotherapy drug efficacy using organoids model of colorectal cancer
10.16352/j.issn.1001-6325.2025.04.0456
- VernacularTitle:利用结直肠癌类器官模型评估化疗药物药效
- Author:
Yuehong GUO
1
;
Fanqi ZHOU
;
Xi WU
;
Guannan ZHANG
;
Fang WANG
;
Jia YU
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系,北京 100005
- Keywords:
colorectal cancer organoids;
drug sensitivity
- From:
Basic & Clinical Medicine
2025;45(4):456-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish human colorectal cancer(CRC)organoids and to evaluate the efficacy of chem-otherapy drugs.Methods Patient-derived CRC cells were cultured to form organoids.The CRC organoids and origi-nal tissues were stained with molecular markers of CRC immunohishtochemically.CRC organoids were used to test drug sensitivity and different concentrations of chemotherapy drugs 5-fluorouracil,oxaliplatin and irinotecan were given respectively;Organoid activity before and after drug treatment was measured by 3D cell viability assay.Results The patient-derived organoids(PDO)from 5 CRC tissues were successfully established.The expression of CK20,Ki67 and Villin proteins was similar in organoids and in original tumor.The organoids retained histologcial features similar to those of the original tumors.Different PDO showed differential sensitivity to different chemothera-py drugs.Conclusions CRC-PDO can dispaly their different sensitivities to different chemotherapy drugs,and could provide valuble reference for personalized treatment for CRC patients.
