Mannitol promotes the apoptosis of human lung squamous carcinoma cell line NCI-H292
10.16352/j.issn.1001-6325.2025.03.0336
- VernacularTitle:甘露醇促进人肺鳞癌细胞系NCI-H292凋亡
- Author:
Huangyi LI
1
;
Daohang HUANG
;
Wenyu ZHANG
;
Dingming HUANG
;
Qichun HUANG
Author Information
1. 广西医科大学附属肿瘤医院 药剂科,广西 南宁 530021
- Keywords:
mannitol;
lung squamous cell carcinoma;
apoptosis;
Bcl-2;
Bax
- From:
Basic & Clinical Medicine
2025;45(3):336-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 20%mannitol injection on the proliferation and apoptosis of hu-man lung squamous carcinoma cell line NCI-H292 and its possible mechanisms.Methods NCI-H292 cells were incubated with 20%and lower concentrations of mannitol.Cell proliferation and viability were detected by CCK-8 assay.Cell apoptosis was detected by flow cytometry and the expression of mitochondrial apoptosis pathway-related proteins Bcl-2 and Bax was detected by Western blot.Results Compared with the control group,mannitol inhibited cell proliferation in a dose-and time-dependent manner(P＜0.05),20%mannitol could induce cell ap-optosis(P＜0.05).The expression level of Bcl-2 was significantly reduced,while that expression level of Bax was significantly increased in cells incubated with 20%mannitol(P＜0.05).Conclusions Clinically used 20%man-nitol injection can induce apoptosis of lung squamous carcinoma cell line NCI-H292.Its mechanism is related to mi-tochondrial pathway-related proteins Bcl-2 and Bax.
