Up-regulation of lncRNA IGF2-AS expression promotes progression of colorectal cancer
10.16352/j.issn.1001-6325.2025.03.0323
- VernacularTitle:LncRNA IGF2-AS表达上调促进结直肠癌的进展
- Author:
Lijiao CUI
1
;
Xiu WU
;
Zhiwei GU
;
Yu ZHOU
;
Caiyuan YU
Author Information
1. 广东医科大学附属医院 消化内科,广东 湛江 524001
- Keywords:
colorectal cancer;
long non-coding RNA;
insulin like growth factor 2 antisense(IGF2-AS)
- From:
Basic & Clinical Medicine
2025;45(3):323-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of lncRNA insulin growth factor 2 anti-sense IGF2-AS in colorectal cancer;To verify the biological function of IGF2-AS in cells and to screen protein mole-cules regulated by IGF2-AS.Methods Using bioinformatics to predict the expression and clinical significance of IGF2-AS in colorectal cancer;Using RT-qPCR to detect the expression of IGF2-AS mRNA;Using small interfering RNA to knockdown IGF2-AS;Using CCK8,Transwell assay and flow cytometry to detect proliferation,migration,and apoptosis,respectively;Using label free quantitative proteomics to screen for factors regulated by IGF2-AS;Using databases such as GenCards,OMIM,TTD,and proteomics results screening for core targets regulated by IGF2-AS in colorectal cancer.Results Bioinformatics analysis showed that IGF2-AS was significantly upregulated in colorectal cancer(P＜0.001),its expression might be related to TNM stage(P＜0.01)and the presence of lymph node metastasis(P＜0.001);RT-qPCR results showed that as compared to the control group,the expression level of IGF2-AS mRNA was significantly up-regulated in colorectal cancer tissues(P＜0.01)and most colon cancer cells(P＜0.000 1);Clinical pathological data indicated that the expression of IGF2-AS was potentially asso-ciated with the lymph node metastasis(P＜0.05);The proliferation rate and migration rate of colorectal cancer cells transfected with IGF2-AS siRNA were significantly reduced(P＜0.000 1),and the apoptosis rate was signifi-cantly increased(P＜0.05);There were 13 proteins including KRAS,TCF7L2 and CASP3,that were potentially core targets regulated by IGF2-AS.Conclusions IGF2-AS is significantly up-regulated in colorectal cancer and may play a cancer promoting role in colorectal cancer development.
