Establishment of a zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology

VernacularTitle:利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除品系
Author: Chunyu JIA ¹ ; Yan SHEN ; Gang CHEN ; Ke ZHENG ; Yan QIN ; Xuemei LI
Author Information

1. 中国医学科学院北京协和医学院北京协和医院肾内科,北京 100730
Keywords: map3k15; CRISPR/Cas9 gene editing technology; zebrafish; kidney development
From: Basic & Clinical Medicine 2025;45(3):290-297
CountryChina
Language:Chinese
Abstract: Objective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases.Methods 1)Analyzing the expression pattern of map3k15 in zebrafish;2)Establishing a homozygous map3k15 knockout zebrafish line using CRISPR/Cas9;3)Observing the phenotypic effects of map3k15 deficiency in ze-brafish.Results 1)map3k15 was expressed in the zebrafish pronephros,and map3k15/map3k15 protein was found highly conserved across species;2)map3k15-/-mutant zebrafish model was constructed with+2 bp mutation and+1 bp mutation;3)map3k15 mutant zebrafish exhibited edema in yolk sac,pericardium and head during embryonic development.Conclusions A homozygous zebrafish map3k15 knockout line is successfully developed which may provide an important model for future research on the role of map3k15 in renal development and disease.