Vitamin D reduces inflammatory response in hypertensive mice with early renal injury
10.16352/j.issn.1001-6325.2025.02.0176
- VernacularTitle:维生素D降低高血压伴早期肾损伤小鼠的炎性反应
- Author:
Junshi ZHANG
1
;
Haiming LIU
;
Xi CHEN
Author Information
1. 新疆医科大学第一附属医院 高血压科,新疆 乌鲁木齐 830000
- Keywords:
hypertension with early renal injury;
vitamin D;
Klotho;
disulfidptosis
- From:
Basic & Clinical Medicine
2025;45(2):176-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the underlying mechanisms of vitamin D treatment improves early-stage renal damage in hypertensive mice.Methods From December 2022 to December 2023,peripheral blood samples were collected from 20 hypertensive patients with early-stage renal injury and healthy controls.ELISA was used to detect interleukin-6(IL?6),C?reactive protein(CRP)and reactive oxygen species(ROS).RT?qPCR and Western blot were utilized to measure the expression of Klotho and disulfidptosis?related proteins[glucose transporter 1(GLUT1),glucose transporter 3(GLUT3)and solute carrier family 7 member 11(SLC7A11)].A mouse model of hypertension with early?stage renal damage was established and treated with vitamin D.Kidney tissue samples were collected for HE staining and ELISA to assess inflammatory factors,and RT?qPCR and Western blot were used to examine the expression of Klotho and disulfidptosis?related proteins.Results Compared to the control group,the level of IL?6,CRP and ROS was significantly increased in the hypertensive patients with early?stage renal damage(P<0.05).The expression of GLUT1 and GLUT3 was significantly increased,while the expression of Klotho and SLC7A11 was significantly decreased(P<0.05).In the mouse model of hypertension with early?stage renal dam?age,HE staining showed that the level of inflammatory factors in the model group was significantly higher than that in control group.Vitamin D suppressed the level of inflammatory factors,increased the expression of Klotho and SLC7A11 and inhibited the expression of GLUT1 and GLUT3 in the model group(P<0.05).Conclusions Vitamin D treatment effectively improves inflammatory response in hypertensive mice with early renal injury.
