Olfactory domain family protein 3 promotes the proliferation of MYCN-amplified neuroblastoma cell lines in vitro
10.16352/j.issn.1001-6325.2025.02.0168
- VernacularTitle:嗅素结构域家族蛋白3促进MYCN扩增型神经母细胞瘤细胞系体外增殖
- Author:
Yao ZHANG
1
;
Xuan ZHANG
;
Anna JIA
;
Shijia ZHAN
;
Jinxin GUO
;
Yan CHANG
;
Yongli GUO
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 儿科重大疾病研究教育部重点实验室 北京市儿科研究所儿童耳鼻咽喉头颈外科疾病北京市重点实验室,北京 100045
- Keywords:
neuroblastoma;
v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog(MYCN)amplification;
ol-factomedin domain family protein 3;
cell proliferation
- From:
Basic & Clinical Medicine
2025;45(2):168-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function of olfactomedin domain family protein 3(OLFM3)in neuroblastoma(NB).Methods The relationship between the expression of OLFM3 mRNA and v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog(MYCN)amplification status and the prognosis of patients in NB clin-ical samples were clarified by using R2 Genomics Analysis and Visualization Platform.Depmap database was used to examine the expression level of OLFM3 in different tumors cell lines and to identify the correlation between OLFM3 expression and MYCN amplification status in various NB cell lines.RT-qPCR and Western blot were used to detect the knockdown level of OLFM3.Cell proliferation was monitored using crystal violet staining and real?time cellular analysis.The colony formation ability of NB cells was assessed using colony?forming unit assay.Results Analysis of R2 database revealed higher level of OLFM3 expression in MYCN?amplified NB clinical samples(P<0.001).Patients with high OLFM3 expression showed a significantly lower overall survival probability compared to those with low OLFM3 expression(P<0.05).Analysis with Depmap database revealed that the expres?sion level of OLFM3 was higher in NB than that in other kind of tumor.The expression level of OLFM3 was signifi?cantly higher in the MYCN?amplified cell lines than in the MYCN?non?amplified cell lines(P<0.01).In MYCN?am?plified NB cells,knockdown of OLFM3 inhibited cells proliferation(P<0.001)and colony formation(P<0.001),but there was no noticeable changes observed in MYCN?non?amplified cells.Conclusions OLFM3 specifically pro?motes the proliferation of MYCN?amplified NB cells,but has a less effect on MYCN?non?amplified cells,indicating it is a potential biomarker for high?risk MYCN?amplified NB.
