Expression and clinical significance of miR-483-3p in serum of gestational diabetes mellitus patients
10.16352/j.issn.1001-6325.2025.01.0091
- VernacularTitle:miR-483-3p在妊娠期糖尿病患者血清中的表达及临床意义
- Author:
Lina ZHANG
1
;
Mengtao JIA
;
Zeyun SUN
;
Jianjun ZHANG
Author Information
1. 山东第二医科大学 临床医学院,山东 潍坊 261035
- Keywords:
gestational diabetes mellitus;
microRNA;
miR-483-3p;
diagnosis
- From:
Basic & Clinical Medicine
2025;45(1):91-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of miR-483-3p in patients with gesta-tional diabetes mellitus(GDM).Methods A total of 100 GDM patients with 24-32 weeks of gestation who under-went routine obstetric examination in Anqiu People's Hospital were selected as the normal glucose tolerance(NGT)group,and a total of 98 healthy pregnant women of the same age of 24-32 gestational weeks were selected as the NGT group.The expression level of miR-483-3p was detected by RT-qPCR,the diagnostic value of miR-483-3p in GDM was analyzed by receiver operating characteristic(ROC)curve,the correlation between the expression levels of miR-483-3p and clinical indicators in the GDM group was analyzed by chi-square test,and the risk factors of GDM were analyzed by Logistic regression.Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-483-3p and autophagy-related protein 7(ATG7).CCK8,flow cytometry and Transwell assays were used to detect the proliferation,apoptosis,and migration and invasion ability of cells,respectively.Results The expression level of miR-483-3p in the serum of patients in the GDM group was significantly higher than that in the NGT group.The expression of miR-483-3p had diagnostic value for GDM.Pre-pregnancy BMI,FBG,FINS,HOMA-IR and TG were significantly correlated with serum miR-483-3p expression.In addition,BMI and TG before pregnancy were risk factors leading to GDM.miR-483-3p regulated HG-treated HTR-8/SVneo cell proliferation,apoptosis,migration and invasion by targeting at ATG7.Conclusions miR-483-3p is involved in the disease pro-gression of GDM and is a potential biomarker of GDM diagnosis.
