Impact of oxygen concentration changes on ANGPTL8 expression of human pulmonary artery endothelial cells
10.16352/j.issn.1001-6325.2025.01.0012
- VernacularTitle:氧浓度变化对人肺动脉内皮细胞中ANGPTL8表达的影响
- Author:
Zongli ZHANG
1
;
Tao LI
;
Jingwen MA
;
Jiaxin ZHANG
;
Xingchao LI
;
Shibing XI
Author Information
1. 十堰市太和医院(湖北医药学院附属医院)儿童疾病研究所,湖北 十堰 442000;十堰市太和医院(湖北医药学院附属医院)儿科 2 病区,湖北 十堰 442000
- Keywords:
pulmonary hypertension;
angiopoietin-like protein 8(ANGPTL8);
endothelial-mesenchymal transition(EndMT);
hypoxia;
hyperoxia
- From:
Basic & Clinical Medicine
2025;45(1):12-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of change in oxygen concentration on the expression of angiopoietin-like protein 8(ANGPTL8)by endothelial cells(HPAECs)of human pulmonary artery,and the role and mecha-nism of ANGPTL8 in pulmonary hypertension(PH).Methods HPAECs were treated under hypoxic and hyperoxic conditions,and the expression level of ANGPTL8 was detected using Western blot and PCR.The changes in endo-thelial-mesenchymal transition(EndMT)and ERK signaling pathway activity were analyzed.Simultaneously,new-born rats were exposed to hyperoxia to develop a bronchopulmonary dysplasia(BPD)model.The expression of ANGPTL8 protein and changes in the ERK signaling pathway in lung tissue were observed.Results Under hypoxic condition,the protein expression of ANGPTL8 in HPAECs was significantly increased accompanied by inhibition of the ERK signaling pathway.ANGPTL8 promoted the EndMT process induced by hypoxia(P<0.05)and silencing the expression of ANGPTL8 resulted in a partial reversal of EndMT.The protein expression of ANGPTL8 was decreased in hyperoxia-exposed HPAECs and rat lung tissues accompanied by the activation of the ERK signaling pathway(P<0.05).Conclusions ANGPTL8 is highly sensitive to the change of oxygen concentration in HPAECs and closely correlated to its expression level and to the activity of ERK signaling pathway.This result suggests that ANGPTL8 may have potential regulatory effects on the development of PH.
