Melatonin promotes anoikis of mouse melanoma cell line B16-F10
10.16352/j.issn.1001-6325.2025.01.0007
- VernacularTitle:褪黑素促进小鼠黑色素瘤细胞系B16-F10失巢凋亡
- Author:
Yuling GAN
1
;
Tingdong LI
;
Libing LIU
;
Yingfen ZHOU
;
Dongsheng PAN
Author Information
1. 甘肃省肿瘤医院 骨与软组织肿瘤一科,甘肃 兰州 730000
- Keywords:
melanoma;
melatonin;
anoikis;
reactive oxygen species
- From:
Basic & Clinical Medicine
2025;45(1):7-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of melatonin on the anoikis of melanoma cells.Methods The drug concentration of melatonin inhibiting melanoma cell line B16-F10 was optimized based on the effect on CCK-8 assay.An anti-anoikis of melanoma cell model was developed and divided it into four groups:The blank control group,the TrkB activator group,the melatonin group and the melatonin+TrkB activator group.Calce-in AM/EthD-1 fluorescence double staining was used to detect the anoikis of melanoma cells.Reactive oxygen spe-cies were detected using the fluorescent probe DCFH-DA.Western blot was used to detect the expression of Nrf2 protein and TrkB protein in each group.Results Melatonin significantly inhibited the proliferation of melanoma cells in a time-and dose-dependent manner with IC50 of 1×10-7 μmol/L.Its inhibitory effect was found to be related to in-duction of anoikis of melanoma cells.Melatonin could upregulate the generation of cellular reactive oxygen species(P<0.05),while addition of TrkB activator antagonized this effect.Melatonin could reduce the expression of Nrf2 protein and TrkB protein in melanoma cells(P<0.05),and the addition of TrkB activator could inhibite the effect of melatonin on the expression of Nrf2 protein and TrkB protein(P<0.05).Conclusions Melatonin can inhibit the pro-liferation of melanoma cell line B16-F10 through the mechanism of inducing anoikis.
