DDX5-Targeting Fragile X Mental Retardation Protein Regulates the Wnt/β-catenin Signaling Pathway to Promote Epithelial Mesenchymal Transition in Breast Cancer
- VernacularTitle:脆性X智力障碍蛋白靶向DDX5调控Wnt/β-catenin信号通路促进乳腺癌细胞上皮-间质转化发生
- Author:
Jia CAO
1
;
Jing WANG
;
Bin SHI
;
Xiaolan MA
;
Weichao WU
;
Nan WANG
Author Information
- Keywords: FMRP; DDX5; EMT; Wnt/β-catenin signal pathway; Breast cancer
- From: Journal of Sichuan University (Medical Sciences) 2024;55(5):1138-1149
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the role of fragile X mental retardation protein(FMRP)in promoting cell migration and epithelial-mesenchymal transition(EMT)in breast cancer(BC)and the potential mechanisms involved.Methods The mRNA and protein expressions of FMRP in MCF-10A,a normal human breast epithelial cell line,and four breast cancer cell lines,including MCF-7,BT474,MDA-MB-231,and HCC1937,were analyzed by RT-PCR and Western blot.The expression of FMRP in BC tissues was measured by immunohistochemistry(IHC).FMRP expression in BC and its relationship with clinical prognosis were analyzed using GEO database.Lentiviral infection and siRNA interference were used to construct FMRP overexpression and interference vectors,respectively,and the human breast cancer cell line MCF-7 was subsequently transfected.A Control group,an interference empty vector group(the NC group),a knockdown vector group(the siFMRP group),an overexpression empty vector group(the Lv-NC group),and an overexpression vector group(the Lv-FMRP group)were set up.The migration and invasion abilities of the cells were assessed by scratch assay and Transwell assay.The expression of EMT markers,including E-cadherin,an epithelial marker,N-cadherin,an mesenchymal markers,vimentin,zinc finger E-box binding homeobox 1(ZEB1),and snail family zinc finger 2(Slug),in the cells of each group was determined by Western blot.The interaction between FMRP and DEAD-box RNA helicase-5(DDX5)protein was analyzed by immunocoprecipitation combined with mass spectrometry(IP-MS).The regulatory effect of FMRP on DDX5 protein expression was assessed using the protein synthesis inhibitor cycloheximide(CHX)and proteasome inhibitor MG132.In addition,transfection with siDDX5 vector was conducted to observe whether DDX5 could reverse the effects of FMRP overexpression on cell migration and EMT.The localization and expression of β-catenin were determined by immunofluorescence staining,and the expression of core markers of Wnt/β-catenin signaling pathway was examined by Western blot.Results FMRP was highly expressed in BC tissues and cells(P＜0.05),and overall survival(OS)and recurrence-free survival(RFS)of the FMRP high expression group were significantly lower than those of the FMRP low expression group(P＜0.05).The migration ability of MCF-7 cells was weakened after FMRP knockdown,while overexpression of FMRP promoted cell migration(P＜0.05).After FMRP knockdown,the expression of E-cadherin was increased,while the expression levels of N-cadherin,vimentin,ZEB1,and Slug were decreased,which inhibited the occurrence of EMT.In contrast,the overexpression of FMRP promoted the EMT process(P＜0.05).FMRP interacted with DDX5 protein and promoted DDX5 protein stability by blocking the ubiquitin-proteasome pathway.DDX5 knockdown reversed the effect of FMRP overexpression to promote cell migration and EMT(P＜0.05),effectively inhibited β-catenin nuclear translocation,and decreased β-catenin nuclear distribution.Furthermore,it was found that the expression of p-β-catenin,GSK3β and Axin2 protein was increased and the expression of C-myc protein was decreased after DDX5 downregulation(P＜0.05).On the other hand,the expression of these proteins was reversed by combined FMRP overexpression(P＜0.05).Conclusion FMRP targets DDX5 and promotes BC cell migration and EMT via the activation of the Wnt/β-catenin signaling pathway.
