Relationship of Cyclooxygenase-2 and Multidrug Resistance Associated Factors to Chemosensitivities in Gastrointestinal Carcinomas
10.3969/j.issn.1672-173X.2010.01.029
- VernacularTitle:胃、大肠癌COX-2和多药耐药相关因子表达与体外化疗药敏性的关系及意义
- Author:
Bi-Bo TAN
1
;
Yong LI
;
Jie HAN
;
Li-Qiao FAN
;
Qun ZHAO
;
Zhen-Chuan SONG
;
Dong WANG
Author Information
1. 河北医科大学第四医院
- Keywords:
Gastrointestinal carcinoma;
Cyclooxygenase-2;
Multidrug resistance;
Multidrug resistance associated factors;
Chemosensitivity test
- From:
Journal of Sichuan University (Medical Sciences)
2010;41(1):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of cyclooxygenase-2(COX-2),p-glycoprotein(P-gp),glutathione S-transferase-πCGST-π),and topoisomerase Ⅱα(TopoⅡα)to chemosensitivities in gastrointestinal tract carcinomas.Methods The tumor tissue samples were collected from 84 specimens of gastrointestinal carcinomas.The expressions of COX-2,P-gp,GST-π,and Topo Ⅱα were determined immunohistochemically.The chemosenisitivity of each sample to 9 drugs were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Results The positive rates of COX-2,P-gp,GST-π and Topo Ⅱα were 48.8%,76.2%,78.6%and 66.7%respectively.The expression of COX-2 was correlated with the expression of P-gp and Topo Ⅱα significantly(r=0.287,0.256,both P＜0.05).In terms of relationships of four MDR-related factors expression to inhibition rate of tumor cells,the inhibition rates of PTX,eADM and OPT in COX-2 strong expression group were significantly lower than those in COX-2 weak expression group(t=2.21,3.11,2.09;all P＜0.05).The inhibition rates of PTX,OXA and DDP in P-gp strong expression group were lower than those in weak group 0=2.54,2.47,2.05;all P＜0.05).There were lower inhibition rates for 5-Fu in GST-π strong expression group(t=2.13,P＜0.05),and higher inhibition rates for VCR,PTX and eADM in TopoⅡα strong expression group(t=-2.29,-2.12,-2.26;all P＜0.05).Conclusions The overexpressions of MDR-related factors in gastrointestinal carcinomas were only associated with the chemosensitivity to some of the chemotherapeutic agents,but not all.MDR related factors may be not specific and accurate predictors for the clinical chemosensitivity.
