The Expression of Basigin mRNA in Early Ischemic Myocardium and Non-ischemic Myocardium

VernacularTitle:Basigin mRNA在早期缺血心肌和非缺血心肌的表达变化
Author: Yun LIU ¹ ; Lin-Bo GAO ; Wei-Bo LIANG ; Xin-Min PAN ; Yan-Yun WANG ; Hui XUE ; Tian-Yi CHEN ; Lu-Shun ZHANG ; Yi ZHU ; Lin ZHANG
Author Information

1. 四川大学
Keywords: Basigin mRNA; Early ischemic myocardium; Non-ischemic myocardium; Real-time; PCR; Rat
From: Journal of Sichuan University (Medical Sciences) 2010;41(1):81-84
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of Basigin mRNA in early ischemic myocardium(EIM)and non-ischemic myocardium(NIM)in rat.Methods Real-time polymerase chain reaction(PCR)technique was applied for detecting Basigin mRNA andβ-actin expression in EIM and NIM at 0 rain,15 min,30 min,and 60 min.Results No significant differences of the Basigin mRNA expression in EIM and NIM between 0 min and sham operation(SO)or non-operation group(P＞0.05)were observed.Basigin mRNA expression in EIM significantly decreased in 15 min,30 min,and 60 min group compared with that of 0 min group,respectively(P＜0.001).However,we failed to find any significant difference in NIM and NO from 0 min to 60 min.Basigin mRNA expression in EIM decreased significantly compared with that of in NIM(P＜0.05)after myocardial ischemia for 30 min.Conclusion Basigin was involved in myocardium pathophysiology process after myocardial ischemia for 30 min,indicating that Basigin may be identified as a predictor of early myocardial ischemia in forensic medicine.