Toxic Effect of Immunosuppression Agent Everolimus on Insuloma Cells and Pancreatic Islets of SD Rat
10.3969/j.issn.1672-173X.2010.01.015
- VernacularTitle:免疫抑制剂依维莫司对大鼠胰岛细胞的毒性作用
- Author:
Xi JIN
1
;
Cong CONG
;
Yu YUAN
;
Gang MAI
;
Ling-Ling WEI
;
You-Nan CHEN
;
Jing-Qiu CHENG
;
Yan-Rong LU
Author Information
1. 四川大学华西医院
- Keywords:
Everolimus;
Immunosuppression agent;
Islet Toxicity
- From:
Journal of Sichuan University (Medical Sciences)
2010;41(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of immunosuppression agent Everolimus on the viability and function of insuloma cells(INS-1)and pancreatic islet cells cultured in vitro.Methods INS-1 cells and islets were treated with a series of concentrations of immunosuppression agents(Everolimus,Cyclosporin A,Sirolimus and Mycophenolate Mofetil).The viability of INS-1 cells and rat pancreatic islets were determined with MTT and the function of INS-1 cells and rat pancreatic islets was evaluated with Glucose-stimulated insulin secretion assay.Results Above the clinical blood concentration,the inhibition rate of islet cell proliferation in the high concentration group of Everolimus and Sirolimus was significantly lower than that of Cyclosporin A and Mycophenolate Mofetil group(P＜0.05);Everolimus in the blood drug level,like other immunosuppressive agents,can inhibit the function of insulin secretion,and the stimulation index of each group was no significant difference.Conclusion Compared to Mycophenolate Mofetil and Cyclosporin A,Everilimus and Sirolimus demonstrate lower toxicity effect on INS-1 cells and rat pancreatic islets in vitro and Everolimus is expected as a new type of immunosuppressive agent used in clinical islet transplantation.
