Valproic Acid Induce the Expression of MICA Antigen in Human Lung Cancer Cells and Promote the NK Killing Effects on the Cells
10.3969/j.issn.1672-173X.2010.01.003
- VernacularTitle:丙戊酸钠诱导人肺癌细胞MICA表达上调增强NK细胞对其杀伤作用的研究
- Author:
Jun RU
1
;
Dan LONG
;
Wei WANG
;
Tao CHOU
;
Zhi-Guo ZHANG
;
Xin-Jian LI
;
Bing FANG
;
Wu YUAN
;
Yong LUO
;
Jing ZHOU
Author Information
1. 四川省成都市第三人民医院
- Keywords:
Valproic acid;
Lung carcinoma cell;
MICA;
NK cells
- From:
Journal of Sichuan University (Medical Sciences)
2010;41(1):10-14,28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of valproic acid(VPA)on MICA gene expression in human lung carcinoma cell lines:A549,SP-CA-1,NCIH446.Methods Different concentration of VPA were applied to treat A549,SP-CA-1,NCIH446 cells respectively and the untreated cells as the control.The effect of the VPA on the cells were observed.Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA.protein level of MICA gene in the three cell lines treated with 1.50 mmol/L and 3.00 mmol/L VPA for 4 days.under the concentration of VPA from 0.75 to 12.0 mmol/L.The viability of lung cancer cells after NK effect in the VPA(+)and VPA(-)treatment were evaluated with LDH method.Results The inhibition effects were observed when the VPA concentration was 6.0 mmol/L above compared to control group,VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentrationdepended pattern.Conclusion VPA induced MICA expression,NK killing effect in the VPA(+)treatment lung cancer cells was significantly stronger than that of VPA(-)condition,which implied a new mechanism of anticancer method and could be a new approach to cancer therapy.
