The Optimal Concentration for the Protective Effect of Emulsified Isoflurane on the Neonatalrat Cardiac Myocytes Hypoxia/Reoxygenation Injury of Primary Culture
10.3969/j.issn.1672-173X.2009.06.024
- VernacularTitle:乳化异氟醚对乳鼠原代培养缺氧/复氧损伤心肌细胞保护效应的最适浓度研究
- Author:
Meng-Chang YANG
1
;
Yu-Pei CHEN
;
De-Jun CAO
;
Qian XU
;
Pan LI
Author Information
1. 四川省医学科学院四川省人民医院
- Keywords:
Emulsified isoflurane;
Cardiac myocyte;
Primary culture;
Hypoxia;
Reoxygenation
- From:
Journal of Sichuan University (Medical Sciences)
2009;40(6):1075-1077,1090
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the concentration of emulsified isoflurane (El) for the protective effect on primary cultured neonatal rat hypoxia/reoxygenation (H/R) cardiac myocytes. Methods To prepare the H/R injury model on the basis of in-vitro neonatal rat cardiac myocytes culture and divide them into 13 groups at random, namely, normal control group (N group), H/R group, H/R+fat emulsion group (F group) , the one, two, three, four, five, six, seven, eight, nine and ten times of 0. 28 mmol/L El designated as EI1-EI10 group. The supernatants of cell culture from each group were detected for lactate dehydrogenase (LDH) activity and the level of cardiac troponin-I (cTnl). The cellular homogenates of each group were prepared for the detection of superoxide dismutase (SOD) and malondialdehyde (MDA). Inverted microscope was applied to observe the characteristics of cardiac myocytes growth and changes of its forms. Results Compared to N group, the LDH. MDA and cTnl of others all increased (P<0. 05) and SOD decreased (P<0. 05). Compared to H/R group, LDH, MDA and cTnl of each El dose group decreased significantly (P<0. 05), but SOD increased (P<0. 05). Compared to EL. group, the LDH, MDA and cTnl in the other groups of El increased (P<0. 05) and the SOD decreased (P<0. 05). As the El concentration (increasing by multiple) increased, the LDH, MDA and cTnl in the El1 to EI6 group decreased gradually and SOD gradually increased (P<0. 05), the LDH, MDA and cTnl in the EL to EI10 group increased gradually and SOD gradually decreased (P<0. 05). Conclusion El has the protective effect on the neonatal rat cardiac myocytes with H/R injury, the optimal concentration for the protective effect on cardiac myocytes is 1. 68 mmol/L, and its mechanism may be associated with its anti-oxidation effect.
