Study on the Immunotoxicity of Ribosome Inhibiting Protein of Momordica Charantia

VernacularTitle:苦瓜蛋白RIP的免疫毒性研究
Author: Li WAN ¹ ; Yan-Fa MENG ; Fu-Bing SHEN ; Jue-Cun ZHENG ; Nian-Hua DENG ; Li-Shi ZHANG
Author Information

1. 四川大学
Keywords: Ribosome inhibiting protein of momordica charantia; Acute toxicity; Immunotoxicity
From: Journal of Sichuan University (Medical Sciences) 2009;40(6):1033-1037
CountryChina
Language:Chinese
Abstract: Objective To separate and purify ribosome inhibiting protein (RIP) from Momordica charantia (bitter melon) seeds and to evaluate its acute toxicity and immunotoxicity in animal. Methods Ion exchange chromatography and gel filtration chromatography were applied in the separating and purifying of RIP from Momordica charantia seeds. Then the acute toxicity testing of RIP in mice was conducted to obtain its half lethal dose (LD_(50)). Active systemic anaphylaxis (ASA) test in guinea pig and passive cutaneous anaphylaxis test (PCA) in rat were performed to evaluate its immunotoxicity. Results The LD_(50) (iv) in mice of RIP was 25. 2 mg/kg in ASA. guinea pigs of the higher and lower RIP group all appeared stong allergic responses and most of them died quickly. In PCA. obvious blue dye in skin were observed in SD rats of the RIP group. Conclusion RIP getting from Momordica charantia seeds had a relatively strong immunotoxicity in animals.