Role of Leptin in Hepatic Ischemia/Reperfusion-induced Hepatic Injury
10.3969/j.issn.1672-173X.2009.06.008
- VernacularTitle:瘦素在肝缺血/再灌注诱导的肝损伤中的作用
- Author:
Ji LIN
1
;
Guang-Tao YAN
;
Hui XUE
;
Xiu-Hua HAO
;
Kai ZHANG
;
Lu-Huan WANG
Author Information
1. 解放军总医院
- Keywords:
Leptin;
Reperfusion injury;
Liver;
Adipose tissue;
Recovery of function Homeostasis
- From:
Journal of Sichuan University (Medical Sciences)
2009;40(6):1003-1007,1043
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of leptin after hepatic ischemia/reperfusion (H-I/R) and its effects on H-I/R-induced hepatic injury. Methods A 70% H-I/R model of rats was established. The rats were divided into groups with different reperfusion times and sham-operation group. Radioimmunoassay was applied to measure protein levels of leptin in serum and adipose tissues of the rats. Enzyme-colorimetry was used to detect serum alanine transaminase. Hematoxylin-eosin staining and immunohistochemistry were applied to investigate pathological variations and protein expressions of leptin in livers, respectively. RT-PCR was used to detect leptin mRNA expressions in adipose tissues and livers. Results Compared with the sham-operation group, serum leptin increased significantly in the 60 min ischemia/360 min reperfusion (I60'R360') group; protein level of leptin in adipose tissues increased significantly in the 160 'R60' group i serum alanine transaminase increased significantly in all of the four reperfusion groups; protein expressions of leptin in livers increased significantly in the 160'R60' and I6O'R240' groups; leptin mRNA expression in adipose tissues decreased significantly in the I6CR150' groups leptin mRNA expression in livers increased significantly in the 160'R60' group; leptin mRNA expressions in livers decreased significantly in the I60'R150', I60'R240' and I60'R360' groups. Pathological investigation showed that hepatic impairments at the early phase of H-I/R were more serious. The impairments at the later phase lessened gradually. Conclusion The change of leptin expressions after H-I/R may be a protective factor to withstand H-I/ R-induced hepatic injury.
