Electrophysiologic Characteristics of Ito in Myocytes from Cardiac Koch Triangle
10.3969/j.issn.1672-173X.2009.06.005
- VernacularTitle:Koch三角心肌细胞瞬间外向钾电流电生理学特征研究
- Author:
Fu-Xian REN
1
;
Xiao-Lin NIU
;
Zhen-Hua HAN
;
Yan OU
;
Feng-Dong LING
;
Shi-Sheng ZHOU
;
Ya-Jie LI
Author Information
1. 河南省濮阳市油田总医院
- Keywords:
Arrythmia;
Ito;
Patch clamp;
Ion channel;
Dynamics
- From:
Journal of Sichuan University (Medical Sciences)
2009;40(6):988-993
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the electrophysiologic characteristics of ho in myocytes from cardiac Koch triangle. Methods Patch clamp technique was employed to investigate the I-V and D-V relation, steady-state activation and inactivation kinetics of Ito from myocytes in Koch triangle of rabbit hearts. Results ① The maximum peak current(pA)and peak current density (pA/pF) at +20 mV in PCCpacing cell), TCCtransitonal cell)α.β, AC (atrial Cell) and PL(purkinje-like cell) cells were different from each other(P<0. 05) ;The cells also had different current density (P<0. 05) except between Tca, TCβ and PL cells(P>0. 05). ②The half activation potential (V_(mlto1/2),mV)of steady state activation among PC, Tca, TCβ, AC and PL were significantly different(P<0. 05); The paired comparison between TCα and TCβ, AC and PL, and TCβ and PC showed significant difference (P< 0. 05);but the differences between TCα and PC, TCβ and AC, and TCβ and PL were not significant(P>0. 05). ③ The half inactivation potential (Vhltol1/2, rnV)of steady state inactivation between Tca and PC, TCα and PL, TCβ and PC, and TCβ and PL demonstrated significant differences(P<0. 05). The differences of Khlto between Tca and TCβ, PC and PL, TCβ and PC, and AC and PL were significant (P<0. 05). Conclusions Ito of cardiac cells from Koch triangle in rabbit hearts are heterogeneous, but relatively specified and distributed in different groups
