Effects of Isocarbophos on the Integrity of Epidermic Cell Membrane of Human Skin
10.3969/j.issn.1672-173X.2001.02.038
- VernacularTitle:水胺硫磷对人皮肤表皮细胞膜完整性的影响
- Author:
Yuanhe YANG
1
;
Mei ZHOU
;
Heng ZHANG
;
Yumin MAO
;
Meihua GUO
Author Information
1. 昆明医学院
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):286-287,246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of isocarbophos, a new and highly effective organophosphorus pesticide on the membranes of keratinocytes. Methods The epidermic cells of human skin cultured in the medium were adopted as a biological material.The activity of lactate dehydrogenase (LDH) was used as an indicator. We tested the activities of LDH and alkaline phosphatase (ALP) in the medium in different isocarbophos dosage conditions. Results It was found that the activity of LDH in the medium decreased with the increase of isocarbophos dosage. The LDH activities of the dosage groups were statistically different from those of the positive and negative groups. The ALP activities were of no significant difference between the groups. Conclusion The above findings reveal that isocarbophos does not damage the membranes of keratinocytes directly, howerer it inhibits the LDH of human skin keratinocytes; thus it is suggested that isocarbophos like most organophosphorus pesticides used widely can pass through the intact human skin and poison the body before the skin is damaged. Therefore, protection during work hours is of paramount importance.
