Correlation of JNK/SAPK Activity and the Production of ICAM-1 on Renal Mesangial Cells in Vitro
10.3969/j.issn.1672-173X.2001.02.035
- VernacularTitle:肾小球系膜细胞的JNK/SAPK活性及其对ICAM-1表达的调控研究
- Author:
Ping FU
1
;
Jianqin WANG
;
Lichuan YANG
;
Songmin HUANG
;
Xianrong LIU
Author Information
1. the First Affiliated Hospital WCUMS
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):277-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the activity of c-Jun N-terminal kinase/stress-activated protein kinase(JNK/SAPK) and assess the correlation between JNK/SAPK activity and ICAM-1 expression on mesangial cells in mesangial proliferative glomerulonephritis(MSPGN). Methods Seven patients with MSPGN and 6 controls were selected for this study.JNK/SAPK activity was detected by immunoprecipitation and western blotting; RT-PCR was used to detect ICAM-1 mRNA expression, and flow cytometry was used to analyze cell surface ICAM-1 expression. Results Either in spontaneous condition or under the stimulus of TNFα, the activity of JNK/SAPK on mesangial cells in vitro in MSPGN was higher than that of the controls (P<0.05); the activity of JNK/SAPK in MSPGN was found to have a positive correlation with the levels of ICAM-1 mRNA and protein. The levels of ICAM-1 mRNA and protein were increased by TNFα stimulation while the levels ICAM-1 mRNA and protein were decreased by DMAP inhibition (P<0.01). Conclusion JNK/SAPK may have been aberrantly activated in MSPGN, which may involve in the overexpression of ICAM-1 in MSPGN.
