Transforming Growth Factor-beta Can Induce Differentiation of Hypertrophic Chondrocytes into Osteoblast-like Cells in Epiphyseal Plate
10.3969/j.issn.1672-173X.2001.02.025
- VernacularTitle:TGF-β诱导骺板肥大区软骨细胞向成骨样细胞分化
- Author:
Fan WANG
1
;
Bensi ZHANG
;
Huijun YANG
;
Aidong LI
;
Ruixiang LI
;
Deming ZHOU
Author Information
1. School of Basic Medical Sciences
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):243-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor-beta (TGF-β) on the differentiation of hypertrophic chondrocytes in epiphyseal plate in order to understand its role in the mechanism of bone and cartilage formation. Methods We observed the effect of TGF-β in varied time and dosage on the differentiation of hypertrophic chondrocytes in embryonic chick femora which were cultured in serum-free medium, using histochemical staining to detect the expression of alkaline phosphatase (ALP) and immunohistochemical staining to detect the expression of type Ⅰcollagen and fibronectin. Results TGF-β enabled the chondrocytes to express type Ⅰcollagen and fibronectin, and it enhanced the expression of ALP in hypertrophic zone. Time-effect and dosage-effect relationships were observed. Conclusion TGF-β can induce the differentiation of hypertrophic chondrocytes into osteoblast-like cells in epiphyseal plate of embryonic chick femora. The inducing effect might be closely related to time and dosage of TGF-β.
